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Image Search Results
Journal:
Article Title: Molecular and Functional Analysis of the lepB Gene, Encoding a Type I Signal Peptidase from Rickettsia rickettsii and Rickettsia typhi
doi: 10.1128/JB.185.15.4578-4584.2003
Figure Lengend Snippet: Western blot analysis of the expression of the R. rickettsii signal peptidase I in E. coli strain IT41. Total proteins from the E. coli cells carrying pTrcHisRR4 or pTrcHisC plasmids, separated on 4 to 12% Tris-glycine precast gel, 1× Tris-glycine-SDS running buffer, transferred to polyvinylidene difluoride membrane was probed with His-Tag monoclonal antibodies by using a WesternBreeze chromogenic immunodetection kit. Lane 1, total proteins from E. coli IT41/pTrcHisRR4; lane 2, total proteins from E. coli IT41/pTrcHisC; and lane 3, total proteins from E. coli IT41. Lane M, Bio-Rad Kaleidoscope prestained markers (carbonic anhydrase, 39.7 kDa; soybean trypsin inhibitor, 32.1 kDa).
Article Snippet: Lane M,
Techniques: Western Blot, Expressing, Immunodetection